The fresh SD region of O. luzonensis charts between eyeless and you may 171M23F to your LG 12 (Tanaka mais aussi al. 2007). We did next linkage studies and you can gotten a couple men recombinants to own this place. You to definitely male got a good recombination breakpoint between OluX2-8 and OluX2-25, therefore the almost every other got a great breakpoint anywhere between OluX3-34 and you can OluX4-six, polishing the fresh SD part to between OluX2-8 and you can OluX4-six ( Contour 1A ).
(A) Genetic and actual map of one’s intercourse-determining places towards X and you can Y chromosomes. step one, seksikkäät Laostian tytГ¶t typical XY men; 2, XX girls; step 3 and you may cuatro, recombinant male. Blue column, places based on new Y-chromosome; reddish, from the X-chromosome. Red-bar, BAC clones; bluish bar, fosmid clones. Black arrows imply forecast family genes. (B) RT–PCR affairs away from PGs (PG1–9) from the XX and you may XY human body trunk in the 0 weeks after hatching. Yards, dimensions marker.
I constructed a beneficial BAC library regarding an XX fish and you may a fosmid collection of a beneficial YY fish, and we generated actual maps of your own SD section of the X and you can Y chromosomes. This area try covered with a couple of BAC clones (OluBXKN2 and OluBXKN1) to your X-chromosome along with eight fosmid clones (OluFYstep 13-step one, OluFY24-step 1, OluFY18-step one, OluFY3-step 1, OluFY8-step 1, OluFY7-1, and OluFY29-1) to the Y chromosome ( Profile 1A ). The entire nucleotide series was dependent on playing with shotgun sequencing, apart from a repeated area from inside the OluFY3-step one and you may OluBXKN1. Limitation studies away from one another clones displayed the length of the fresh new repetitive part was a similar for chromosomes (investigation not found). Continue reading “Nine genes had been forecast throughout the SD region”